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Education
B.A. Taiwan University Taiwan Forestry (1972)
M.S. Taiwan University Taiwan Forestry (1974)
M.S. University of Minnesota U.S.A. Plant Physiology (1983)
Ph.D. University of Minnesota U.S.A. Plant Physiology (1988)
Current Research of Tsai Yun Lin’s
Laboratory
Plant Environmental Stress and Natural
Herbs
Environmental Stress
Low non-freezing
temperatures severely challenge crop productivity and
quality to tropical or subtropical plants. To uncover the
molecular mechanisms of the inherent
chilling-susceptibility, we perform a comparative gene
expression profiling in seedlings of two mungbean (Vigna
radiata) varieties. Our goal is to explore the
molecular mechanism of the repair of chilling injury and
resumption of plant growth during the recovery process using
the cDNA microarray and qRT-PCR. Low temperature is
required for
Phalaenopsis
aphrodite subsp. formosana to shift from
vegetative growth to reproductive growth; however, chilling
decreases orchid production. It is of interest to study the
physiological function of the genes in response to
environmental changes. Studies on the expression patterns
of stress-induced genes and their physiological roles in
P. aphrodite and V. radiata are undertaken in our
laboratory.
Natural Herb
Recently the healing properties of natural
herbs have received wide public attention and medical
validation in the world. We have studied the regulatory
network of methyl-jasmonate (MeJA)-induced genes in
adventitious root cultures of the medicinal herb
Bupleurum kaoi using a functional transcriptomic
approach with a cDNA microarray and real-time qRT-PCR to
verify changes in expression of the uniESTs. Some of these
MeJA-induced genes have applications in agricultural,
pharmaceutical and food industries. We intend to carry out
further experiments to reveal the physiological functions of
the gene products.
Teaching Activities
Life Science 3121 Plant Physiology
Plant Physiology is an introductory course for presenting the
conceptual advances in the field of plant physiology. Lectures
include essential concepts, transport and translocation of water
and solutes, plant biochemistry and metabolism, as well as plant
growth and development. This course will emphasize the
physiological and biochemical functions of plants.
Life Science 6443 ?Topics in Plant Molecular Biology
This course focuses on several aspects of plant development and
metabolism with particular emphasis on the emerging role of
plant responses to changing environments. Students will present
oral reports and lead discussion on research progress in
specific areas of molecular, cellular, and developmental
biology.
Life Science 6421 - Advanced Molecular Biology (shared)
This course is offered to graduate students and senior
undergraduate students who have taken molecular biology class.
The material contains genetics in bacteria, yeast and
eukaryotes, transcription and translation, the interactions of
individual proteins and RNAs with DNA, and cell growth, cancer
and growth regulation.
Papers Published within last 5 years
Lin, W. Y., Chen, L.
R., and Lin, T. Y. (2008) Rapid
authentication of Bupleurum species using an array of
immobilized sequence-specific oligonucleotide probes. Planta
Med. 74, 464-469.
Chen,
L. R., Markhart, A. H., Shanmugasundaram, S.,
and Lin, T. Y.
(2008) Early developmental and stress responsive ESTs from
mungbean, Vigna radiata (L.) Wilczek, seedlings.
Plant Cell Rep.
27, 535-552.
Chiou, S. J., Yen,
J. H., Fang, C. L., Chen, H. L., and Lin, T. Y. (2007).
Authentication of medicinal herbs using PCR-amplified ITS2
with specific primers. Planta Med. 73, 1421-1426.
Chen, L. R., Chen, Y. J.,
Lee, C. Y., and
Lin, T. Y.
(2007) MeJA-induced transcriptional changes in adventitious
roots of Bupleurum kaoi. Plant Sci. 173, 12-24.
Lee,
H. C., Chen, Y. J., Markhart A. H.,
and
Lin, T. Y. (2007) Temperature effects on systemic
endoreduplication in orchid during floral development. Plant
Sci. 172, 588-595.
Chen, H. C., Lee, H. C.,
Lin, T. Y., and Chen, B. S. (2006) Stabilization control of
small heat shock proteins in cellular chaperone protection
systems. J Chin Inst Eng 29, 361-373.
Chen, Y. J., Chen, L. R.,
Lee, L. C., Liu, W. Y., Lee, C. Y., Chiou, S. J., and Lin,
T. Y. (2005). Industrial applications of Bupleurum kaoi
genes induced by MeJA. International Journal of Applied
Science and Engineering 3, 157-166.
Lee,
H. C. and Lin, T. Y. (2005). Isolation of plant nuclei
suitable for flow cytometry from recalcitrant tissue using a
filtration column. Plant Mol. Biol. Rep. 23,
53-58.
Chen, Y. J., Wu, M. F., Yu,
Y. H., Tam, M. F., and Lin, T. Y. (2004).
Developmental expression
of three mungbean Hsc70s and substrate binding specificity
of the encoded proteins. Plant Cell Physiol. 45, 1603-1614.
Chen, H. C., Lee, H. C.,
Lin, T. Y., Li, W. H., and Chen, B. S. (2004). Quantitative
characterization of the transcriptional regulatory network
in the yeast cell cycle. Bioinformatics 20, 1914-1927.
Lee, H. C., Chiou, D. W.,
Chen, W. H., Markhart, A. H., Chen, Y. H., and Lin, T. Y.
(2004). Dynamics of cell growth and endoreduplication during
orchid flower development. Plant Sci. 166, 659-667.
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